BIOCHEMISTRY

Q: The enzyme used for the mapping of hypersensitive sites in recombinant DNA research is?

(a) :  DNA ligase
(b) :  DNA polymerase I
(c) :  DNase I
(d) :  Polynucleotide kinase
(e) :  None of the above
Answer:  DNase I
Explanation:  

Q: Deficiency of lysyl hydroxylase causes?

(a) :  Alport syndrome
(b) :  Menkes disease
(c) :  Epidermolysis bullosa
(d) :  Osteogenesis imperfecta
(e) :  None of the above
Answer:  Menkes disease
Explanation:  

Q: Which of the following is true regarding Okazaki fragment?

(a) :  Are segments of RNA attached to an RNA initiator component
(b) :  Are related to the leading strand
(c) :  Several Okazaki fragments must be sequentially synthesized for each replication fork
(d) :  Helicase acts on the leading strand to unwind dsDNA
(e) :  None of the above
Answer:  Several Okazaki fragments must be sequentially synthesized for each replication fork
Explanation:  

Q: The most widely used method for determining the purity of a protein is?

(a) :  Isoelectric focusing
(b) :  Polyacrylamide gel electrophoresis
(c) :  Ion exchange chromatography
(d) :  High pressure chromatography
(e) :  None of the above
Answer:  Polyacrylamide gel electrophoresis
Explanation:  

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